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16-101-

3250 Single-Sample Osmometer

The 3250 Single-Sample Osmometer is designed for routine osmolality measurement in pharma, biotech, and food and beverage labs. Providing accurate and precise osmolality measurement, the 3250 combines proven freezing point technology with the versatility of advanced sample processing capabilities in an osmometer that is both simple to operate and easy to maintain.

Highlighted features:
  • Single-sample osmometer
  • Wide operating range of 0-4000 mOsm
  • Gold-standard freezing point technology
  • Meets Pharmacopeia osmolality testing guidelines

Overview

Industry Applications

IN BIOTECH LABS

Maximise biologic yield, quality, and purity by optimising and ensuring the quality and consistency of your buffers, media, product, excipients, and process parameters.

IN FOOD & BEVERAGE LABS

Maintain efficiency while ensuring product quality and process consistency with osmolality testing from formulation to release.

PRODUCT FEATURES

GOLD STANDARD PERFORMANCE

The Model 3250 uses a 200-250 μL sample volume and provides the best accuracy and precision of ANY osmometer. Sample test time is 2-3 minutes depending on the operating range

PROVEN RELIABILITY

The 3250 system incorporates over 50 years of applied technology and expertise in the field of osmometry and is ideal for laboratories seeking greater control, minimal downtime, and higher productivity

EASY TO USE

With a push-button menu-driven display, automatic calibration, and onboard statistical analysis, the 3250 bundles world-class performance into a user friendly package

WIDE OPERATING RANGE

The Model 3250 offers the widest measurement range of any osmometer, delivering accurate and precise measurements from 0-4000 mOsm. Select from 2 calibrated operating ranges depending on your sample type

FLEXIBLE REPORTING OPTIONS

The 3250 provides an onboard printer, optional bar code scanner, internal memory storage of test results, and the ability to connect to LIS, data management or middleware such as [email protected] Data Cockpit

BACKED BY COMPREHENSIVE SUPPORT

You can purchase any Advanced Instruments osmometer with complete confidence as every instrument is backed by a knowledgeable, experienced, and responsive customer support team.

Technical Specifications

Sample test volume200 to 250μL
Test timeLow range: 2 minutes (approximate)
High range: 3 minutes (approximate)
Sample capacitySingle sample
UnitmOsm/kg H2O
Resolution1 mOsm/kg H2O
RangeLow range: 0 to 2000 mOsm/kg H2O
High range: 0 to 4000 mOsm/kg H2O
Linearityless than ±0.5% from a straight line over calibrated range
Repeatabilitystandard deviation ≤ 2 mOsm/kg H2O between 0 to 400 mOsm/kg H2O; standard deviation ≤ 0.5% of value between 400 to 4000 mOsm/kg H2O
DriftLess than 1 mOsm/kg H2O per month
Temperature effects1Less than 1 mOsm/kg H2O per 5°C (9°F) ambient temperature change
communicationOnbord printer, DTE RS-232 Serial Port and optional bar code scanner
support languagesEnglish, French, German, Spanish, Italian, Portuguese, Swedish, Danish, Turkish, Czech, Slovak
Storage temperature-40°C to +45°C (-40°F to +113°F)
Electrical voltage100 to 240 VAC (50/60 Hz)
Power consumption95W
Dimensions (D x W x H)16″ H x 13″ W x 18″ D (40.6 cm x 33.0 cm x 45.7 cm)
Net weight23.0 lb (10.4 kg)
Shipping weight34.0 lb (15.4 kg)
WarrantyOne-year limited warranty on workmanship and all parts except glass, plastic, and part warrantied by their makers
*operating condition -18°C to 35°C (64°F to 95°F); 5% to 80% relative humidity (noncondensing)
*specifications subject to change

Associated Products

Osmometer Calibration Standards

50, 850 & 2000 mOsm
10 x 2mL Ampules

Osmolality Linearity Set

100-2,000 mOsm
5x2x5mL Ampules

Osmometer Calibration Standards and Reference Solutions

SK-3250 BiotechConvenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011)
SKC-3250 ClinicalConvenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011, 3MA028, 3LA085)
3LA011100 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA029Clinitrol™ 290 Reference Solution, 10×5 mL AmpulesView
3LA091900 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA1511500 mOsm Calibration Standard, 10×5 mLView
3LA3013000 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA028Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mLView

Osmometer Control Solutions

3MA028Protinol 3-Level Osmometer Control, 3x3x3 mLView
3LA085Renol 2-Level Osmometer Control, 2x4x3 mLView

Supplies & Accessories

3LA825Sample Tube, Plastic, Box 500
3DA811Heat Transfer Fluid, 1×150 mL
3255User’s Guide
3255SMService Manual
3D2340Air Filters, Disposable, 6/pkg
330016Barcode Scanner
3LA84630 Sample Tube Rack
FLA835Thermal Printer Paper, 5 rolls

Biotech Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Applications

Resources for applications of using osmolality within biotechnology processes and drug manufacturing, plus product quality assurance within food & beverage.

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

  • Are all of your buffers and media formulated correctly?
  • Does your solution have unexpected components?
  • Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

Upstream

Optimise cell culture conditions to maximize cell density and yield.

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system contributing to optimal cell density and tite.

Media Preparation

Check media composition for optimal cell growth.

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum density and yield.
Downstream

Monitor buffer composition and processes, such as chromatography and filtration, to ensure protein purity and yield.

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.

Chromatography

Maintain the purity of the drug intermediates and identify and process deviations that could compromise product yield.

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimise the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and verify the concentration of API and excipients to meet internal quality and regulatory requirements

Formulation Development

Optimise formulation concentration and stability for high yield in manufacturing.

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content of formulations. Minimize injection site pain.

Filtration and Fill

Key compendial release specification to confirm product stability.

References

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing.  Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

  • Are you looking for ways to increase vector stability?
  • Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

    • how to optimise the physiological range for transfection complexes
    • identifying media needs
    • determining effects of osmotic pressure on the stability of a complex
Upstream

Optimise cell culture conditions and media selection to maximise cell density, size and, ultimately, virus yield.

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system, contributing to optimal cell density and titer. Maximise transfection efficiency for optimal yield.

Media Preparation

Characterise media preparation and composition for optimal cell growth

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell density and yield.
Downstream

Check raw material concentration and perform in-process monitoring of purification and filtration steps to maximise purity, cell/virus stability, and yield.

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.

Chromatography

Maintain the purity of the drug intermediates, protect the viral vector and genetic content within the vector during process, and identify process deviations that could compromise product yield.

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition, dosage, and stability

Formulation Development

Optimize formulation concentration and composition for high yield in manufacturing.

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content (solute concentrations) of formulations. Ensure compatibility of precious product with cryopreservatives.

Filtration and Fill

Key compendial release specification to confirm product dosage specifications.

When the therapeutic you’re developing is cell-based, constant monitoring to optimise growth and ensure consistent culture conditions throughout multiple stages is critical for success.

  • Are your stem cells at highest viability and product quality levels throughout culture and passaging?
  • Are you ensuring a high rate of cell recovery post-thaw?
  • Are your transduction mechanics being optimised?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

Upstream

Characterise media and optimise transduction of cells with lentivirus to optimise cell viability and size.

Cell Line Development

Ensure appropriate trending by monitoring small-scale cell culture for some cell therapies.

Media Preparation

Characterise media formulation and QC raw materials for optimal cell growth.

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell viability.
Downstream

Optimize downstream processing by confirming buffer concentrations and minimizing shock to cells.

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.

Chromatography

Maintain purification conditions to maximise yield and protect cells.

Buffer Exchange

Optimise solution concentrations for UF/DF concentration and buffer exchange to acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition and manage cryopreservation and storage.

Formulation Development

Optimise formulation concentration and composition drug safety.

Final Formulation

Confirm concentration of formulations and ensure compatibility of cells with cryopreservatives.

Formulation and Fill

Key compendial release specification to confirm stability.

Ensure optimal product consistency with quick and accurate osmolality measurement using an Advanced 3250 Osmometer. Designed to support food and beverage formulation and development efforts, our osmometers help maximize resources and ensure consistent product quality.

References

  1. Wesley, J. Sports Hydration: ‘07. 2006 November 2

Comparison Guide

All models support:
  • Pharmacopeia testing guidelines

Workflow Features
Best for labs with daily sample processing needs of:>10/day>10/day1-10/day1-10/day
Sample volume100 µL20 µL20 µL250 µL
Sample introductionAutomated liquid handling sample cup (automated)Sample cupOne-step directSample cup
Operating range (mOsm/kg H2O)0 – 4,0000 – 2,0000 – 2,0000 – 4,000
Test time (seconds)1809090120
Compliance Support Features
Supports 21 CFR part 11 & EU Annex 11 compliance
Supports Pharmacopeia testing guidelines compliance
Instrument Features
Integrated barcode scanner
Printer
Multi-language touchscreen display
User name & password compatibility
Multi-sample capacity
Automated cleaning between samples
Automated sample pipetting
Temperature control methodDry cooling (No heat transfer fluid)Dry cooling (No heat transfer fluid)Dry cooling (No heat transfer fluid)Heat-transfer fluid
Data Management Features
TCP/IP
USB
Webserver
OPC-UA, Network share,
Database backup
DTE RS-232, Barcode port
Part #A2OOsmoTECHPROOsmoTECH3250
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  1. Sold separately
  2. Printer (dot-matrix) sold separately
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Educational Resources

Brochure

Advanced 3250 Single Sample Osmometer

Education

Osmolality as a concentration measurement for key buffers in bioprocessing

Application Note

Osmolality as a valuable tool in the cryopreservation of advanced therapies