3250 Single-Sample Osmometer

The Advanced® Model 3250 is a single-sample osmometer designed to provide fast, accurate test results using a 200-250 µL sample. It combines proven freezing point technology with the versatility of advanced sample processing capabilities in an osmometer that is both simple to operate and easy to maintain. It is ideally suited for routine osmolality testing in the clinical laboratory, and also for research applications in pharmaceutical, academic, and industrial laboratory settings.


Gold standard performance

The Model 3250 uses a 200-250 μL sample volume, provides the best accuracy and precision of ANY osmometer. Sample test time is 2-3 minutes depending on the operating range

Proven reliability

The 3250 system incorporates over 50 years of applied technology and expertise in the field of osmometry and is ideal for laboratories seeking greater control, minimal downtime, and higher productivity

Easy to use

With features including a menu-driven display with push-button design, automatic calibration, and onboard statistical analysis, the 3250 combines world-class performance in a user friendly package

Wide operating range

The Model 3250 offers the widest measurement range of any osmometer of 0-4000 mOsm. Select from 2 calibrated operating ranges depending on your sample type

Versatile sample processing

The 3250’s test parameters can be optimized based on the sample type, making it an ideal research osmometer for analyzing a wide variety of complex aqueous mixtures

Flexible reporting options

The 3250 provides an onboard printer, optional bar code scanner, internal memory storage of test results, and the ability to connect to LIS or data management systems

Products & Supplies

Available Models

3250 Model 3250 Osmometer

Osmometer Calibration Standards and Reference Solutions

SK-3250 Biotech Convenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011)
SKC-3250 Clinical Convenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011, 3MA028, 3LA085)
3LA011 100 mOsm Calibration Standard, 10x5 mL Ampules View
3LA029 Clinitrol™ 290 Reference Solution, 10x5 mL Ampules View
3LA091 900 mOsm Calibration Standard, 10x5 mL Ampules View
3LA151 1500 mOsm Calibration Standard, 10x5 mL View
3LA301 3000 mOsm Calibration Standard, 10x5 mL Ampules View
3LA028 Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mL View

Osmometer Control Solutions

3MA028 Protinol 3-Level Osmometer Control, 3x3x3 mL
3LA085 Renol 2-Level Osmometer Control, 2x4x3 mL

Supplies and Accessories

3LA825 Sample Tube, Plastic, Box 500
3DA811 Heat Transfer Fluid, 1x150 mL
3255 User's Guide
3255SM Service Manual
3D2340 Air Filters, Disposable, 6/pkg
330016 Bar Code Scanner
3LA846 30 Sample Tube Rack
FLA835 Thermal Printer Paper, 5 rolls


Brochure, 3250Download (PDF)
ControLine™ ProductsDownload (pdf)
Effects of Hemolysis, Icterus, and Lipemia on Serum Osmolality Results using the Advanced Model 3250 Single-Sample OsmometerDownload (pdf)
Osmometry – The Rational Basis for use of an Under-appreciated Diagnostic ToolDownload (pdf)
Osmometry RevisitedDownload (pdf)
Performance Evaluation of Commercially Available Osmometer Control SolutionsDownload (pdf)
Refrigerated and Room Temperature Storage Stability of Serum Osmolality MeasurementsDownload (pdf)
Refrigerated and Room Temperature Storage Stability of Urine Osmolality MeasurementsDownload (pdf)
Sports Hydration: ’07Download (pdf)
The Physical Chemistry, Theory and Technique of Freezing Point DeterminationsDownload (pdf)
The Value of Osmolality in Emergency MedicineDownload (pdf)
The Value of Osmolality in NephrologyDownload (pdf)
The Value of Osmolality TestingDownload (pdf)
Using Osmometry for Water – Electrolyte Balance Experiments in the Instructional LaboratoryDownload (pdf)
Utilization of Controls, Materials, and Osmometry in a Clinical Setting Offers Quality BenefitsDownload (pdf)
Water Metabolism: Made EasierDownload (pdf)

Workflow Graphic

Testing osmolality is an essential parameter for process control and QC – just one of the many complex operational steps in biopharma manufacturing dedicated to producing a product of predetermined yield, purity and quality.

  • Guarantee quality, potency and consistency during media development and raw material management.
  • Monitor cell culture and fermentation to ensure optimal cell health, and consequently, high product quality and yield.

Ensure precise buffer preparation and prove complete buffer exchange to improve purification efficiency.

Safeguard your biologics as it passes through storage, reconstitution and injection.

Ensure that the final product meets your quality standards and release criteria.